Pineapple mealybug wilt-associated viruses 1, 2, and 3 are associated with mealybug wilt disease of pineapple in Peru

Abstract Mealybug wilt of pineapple (MWP) is the most important disease worldwide. During months September and November 2020, production fields cultivar Hawaiiana hybrid MD-2 in Satipo Chanchamayo provinces Peru, showed typical symptoms MWP. Based on symptoms, incidence level ranged between 70 90%. Symptomatic asymptomatic foliar samples were collected tested by RT-PCR using primers specific for mealybug virus (PMWaV) 1, 2, 3. Selected amplified fragments sequenced analyzed with bioinformatics tools. PMWaV-1, 3 detected symptomatic both cultivars but not samples. PMWaV-1 a nucleotide identity 91.70% from Thailand; PMWaV-2, 99.81% PMWaV-2 PMWaV-3, 99.09% PMWaV-3 Cuba. Mixed infections also cultivars. To our knowledge, this first report 3, associated Peru.